other:
Mass Spectrometry:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Electrophoresis:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Bicinchoninic Acid Protein Assay:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Multiplex Assay:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Dissolution:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Chromatography:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Lysis:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
High Performance Liquid Chromatography:Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Main reagents and apparatus Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
Article Title: The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis
Article Snippet: .. Easy nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiskan FCMicroplate Photometer (Thermo Scientific), centrifuges (Eppendorf 5430R), Concentrator Plus/Vacufuge (EppendorfConcentrator Plus), Electrophoresis (GE Healthcare EPS601), MP Fastprep-24 Automated Homogenizer (MP Biomedicals), ultrasonic liquid processors (ScientzJY92-II, Ningbo), electric thermostatic incubator (Jinghong GNP-9080, Shanghai), vortex (QiTeQT-1, Shanghai), electronic balance (METLER TOLED AL104), MaxQuant 1.3.0.5 (Max Planck Institute of Biochemistry in Martinsried, Germany), Perseus 1.3 (Max Planck Institute of Biochemistry in Martinsried, Germany), glycerol (G0854, Sangon/500 ml), bromophenol blue (161-0404, Sangon), SDS (161-0302, Bio-Rad), urea (161-0731, Bio-Rad), Tris (A6141, Sigma), DTT (161-0404, Bio-Rad), iodoacetamide (IAA, 163-2109, Bio-Rad), KH 2 PO 4 (10017618, Sinopharm), KCl (10016318, Sinopharm), HCl (10011018, Sinopharm), BCA Protein Assay Kit (P0012, Beyotime), Bovine Serum Albumin (BSA) (A0332-25G, Sangon), NH 4 HCO 3 (A6141, Sigma), trypsin (317107, Promega), formic acid (FA, 06450, Fluka), trifluoroacetic acid (TFA, T6508, Sigma), acetonitrile (CAN, I592230123, Merck), 10-kDa ultrafiltration tube (Sartorius), C18 cartridge (66872-U, Sigma), Multiple Affinity Removal LC Column-Human 14/Mouse 3 (Agilent), iTRAQ Reagent-4/8 plex Multiplex Kit (AB SCIEX), dissolution buffer (AB SCIEX), SCX chromatography column: Polysulfoethyl 4.6 × 100 mm column (5 μm, 200 Å) (PolyLCInc, Columbia, MD, USA), C18 Trap Column: Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, 3 μm, 100 Å, C18 Analytical Column: Thermo Scientific EASY Column, 10 cm, ID 75 μm, 3 μm, C18-A2, 5 × loading buffer: 10% SDS, 0.5% bromophenol blue, 50% glycerol, 500 mM DTT, 250 mM Tris-HCl, pH 6.8, SDT lysis buffer: 4% SDS, 100 mM Tris-HCl, 1 mM DTT, pH 7.6, UA buffer: 8 M urea, 150 mM Tris-HCl, pH 8.0, SCX Buffer A: 10 mM KH 2 PO 4 pH 3.0, 25% CAN, SCX buffer B: 10 mM KH 2 PO 4 pH 3.0, 500 mM KCl, 25% CAN, HPLC Buffer A: 0.1% FA, HPLC buffer B: 0.1% FA, 84% CAN. ..
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